In this panel, you can compare gene/signature expression level between patients with and without key hotspot mutations.

GEPIA performs ANOVA or Wilcoxon rank-sum test for differential expression analysis with hotspot mutation. Only significant results will be provided (p < 0.1). Meanwhile, you can compare expression correlation between your query gene and hotspot gene in mutant or non-mutant cases by click 'plot' button. Spearman's r was used in Correlation analysis. Only primary tumor expression level was included in this function. The criteria for hotspot mutations are mutation frequency > 0.05 and occurrence in at least 10 samples.

Parameters:

• Gene: Input a gene/isoform or gene signature A of interest.
• Methods: Select ANOVA or Wilcoxon for differential analysis.
• log2 transformed: Select whether use log2(TPM+1) as expression abundance.
• Hotspot Mutation Type: Select the type of mutation. Gene level means any nonsilent mutation in the gene. Amino acid level means changes at specific amino acid site (HGSV protein-level).
• Datasets Selection: Select one or multiple cancer types of interest in the "Dataset Selection" field and click "add" to build dataset list in the "Datasets" field.
• Plot Color: Choose group color to plot differential expression boxplot and correlation scatter plot.

Results:

• The table shows the significant results of differential expression between hotspot mutant and wildtype cases. Click the button beside mutation to plot gene/signature expression distribution of mutant and wildtype groups, as well as the expression correlation with the hotspot gene.